Stem cell research - one of science's most promising fields - could help medicine to develop new cell based therapies, to discover new drugs and to extend scientific knowledge. But one of the challenges in culturing cells is to maintain cell viability and to achieve efficient differentiation. Gelatin has been shown to be a material in which stem cells can grow and differentiate easily (Nikkhah, et al., 2016). However, the presence of endotoxins in biomaterials can negatively impact viability and differentiation of stem cells even in the presence of trace amounts of endotoxins (Nomura, et al., 2018 and Lieder, et al., 2013). When it comes to cell storage or cell transport, an endotoxin-free environment keeping the cells perfectly alive is also highly desirable. Endotoxin levels higher than 0.05 EU/ml have been found to inhibit osteoblast differentiation (Kadono, et al., 1999) and proliferation of hematopoetic stem cells (Rinehart, et al., 1997).
Also, for organ-on-a-chip applications – for example, for toxicity screening – low endotoxin levels are crucial as they can cause unwanted cell behavior and functionality results (Tarrant, 2010).
INTERESTED TO KNOW MORE?
Read our Scientific poster on the ‘Influence of autoclave sterilization of gelatin on physical properties and endotoxin level and the influence of endotoxin level on endothelial cellular activity’
In a recent poster (Vanhoecke and Olijve, 2018), the influence of autoclave sterilization of gelatin and endotoxin levels on endothelial cellular activity was evaluated. The aim of this study was to determine the influence of autoclave sterilization of gelatin on physical properties and on endotoxin levels. Additionally, the influence of endotoxins on cellular viability of an endotoxin-sensitive endothelial cell line was evaluated.