Cultivo de células madre

Biomaterials for cell culture can foster the development of new cell-based therapies, support tissue engineering, aid the discovery of new drug targets and improve our understanding of cell behavior. Importantly, in vitro tests using human cells and tissue are proving to be an effective alternative to animal testing. 

Gelatin is a highly versatile and reliable biomaterial derived from native collagen, a natural protein found in the extracellular matrix of animals. Its unique properties, including cell recognition sites, excellent biocompatibility, low antigenicity and tunable biodegradability, allow researchers to create intricate three-dimensional microenvironments that encourage cell adhesion, growth, differentiation, migration, signaling and survival.

• We offer a wide choice of purified gelatins with very low levels of endotoxins and impurities. 
• X-Pure^® gelatins include hydrolyzed and non-hydrolyzed grades for 2D cell culture coating, as well as functionalized gelatins such as X-Pure^®GelMA and X-Pure^®GelDAT, each with distinct properties suited to 3D culture applications.
• We understand the importance of adapting gelatin properties to different cell origins, e.g. liver cells need a softer environment than bone cells. All our X-Pure^® gelatins can be customized to meet your specific needs.
• Consistency of our products from batch to batch is essential in our production process, guaranteeing the uniformity and quality our customers expect.
• Our research-grade X-Pure^® gelatin is functionally equivalent to its GMP grade, minimizing the need to revalidate R&D results prior to clinical or non-clinical trials, and helping you speed your biomedical innovation to market.  
• All Rousselot products comply with the regulatory requirements of the major pharmacopoeias, including raw material requirements of the EU Medical Device Regulation (MDR) 2017/745, (IVDR) 2017/746 and US-FDA guidance for (in vitro) medical devices, and come with traceability as required by ISO 22442.

In a number of studies, X-Pure^® has outperformed standard research grade gelatins and coating materials, supporting successful cell culture.

With ultra-low impurity levels, X Pure^® supports the reproduction of human body conditions and paves the way for successful cell growth, differentiation and communication.¹

A recent study demonstrated that Rousselot’s purified GMP-grade gelatins support cell morphology and efficient stem cell proliferation, outperforming standard non-endotoxin purified research grade gelatins and other coating materials in 2D cell culture.²

A recent study led jointly by the University of Twente and Rousselot has shown for the first time that the predictive value of 3D breast tumor-immune cancer in vitro models is significantly impacted by the presence of endotoxins.³ The findings suggest that biomaterials with low endotoxin content could lead to a more accurate representation of the safety and potency of novel therapeutics.

The use of purified biomaterials such as Rousselot’s X-Pure^®, X-Pure^® GelMA or X-Pure^® GelDAT in cell culture and in vitro 3D models is critical in producing a biologically relevant environment, while also helping to reduce the costs, time and ethical concerns associated with drug development.

1. Olijve, J and Vanhoecke, B. 2019. Low Endotoxin Gelatins and Collagens For (Bio)Medical Applications.
2. Poster: Stem cell proliferation on 3D printed discs improved with low endotoxin and low molecular weight gelatin coating by Jos Olijve, Elien Gevaert Rousselot BV, Gent, Belgium. Available at: https://www.rousselot.com/biomedical/media/news/new-study-confirms-that-purity-and-type-of-coating-biomaterials-used-in-2d-cell-culturing-are-crucial-for-efficient-stem-cell-proliferation#:~:text=Click-,here,-to%20view%20the
3. Heinrich, M. A., Heinrich, L., Ankone, M. J. K., Vergauwen, B., & Prakash, J. (2023). Endotoxin contamination alters macrophage-cancer cell interaction and therapeutic efficacy in pre-clinical 3D in vitro models. Biomaterials advances, 144, 213220. https://doi.org/10.1016/j.bioadv.2022.213220
4. Groen WMGAC, Utomo L, Castilho M, Gawlitta D, Malda J, Weeren PRV, Levato R, Korthagen NM. Impact of Endotoxins in Gelatine Hydrogels on Chondrogenic Differentiation and Inflammatory Cytokine Secretion In Vitro. Int J Mol Sci. 2020 Nov 13;21(22):8571.
5. Heinrich, M. A., Heinrich, L., Ankone, M. J. K., Vergauwen, B., & Prakash, J. (2023). Endotoxin contamination alters macrophage-cancer cell interaction and therapeutic efficacy in pre-clinical 3D in vitro models. Biomaterials advances, 144, 213220. https://doi.org/10.1016/j.bioadv.2022.213220