X-Pure GelDAT
What is X-Pure GelDAT?
X-Pure GelDAT® is the world’s first purified Gelatin Desaminotyrosine (also known as Gelatin Hydroxyphenyl Propionic Acid or Gelatin Phloretic Acid) – a modified gelatin that combines the benefits of Rousselot X-Pure gelatins with a phenolic modification inspired by sea-mussel adhesion to wet surfaces. With two different crosslinking cues, X-Pure GelDAT can be stable at body temperature (37°C) and has the versatility to be combined with other modified biomaterials to create more complex structures.
Figure 1: The phenolic side groups can be crosslinked with two different cues: A) photo crosslinked in the presence of a photo initiator and UV or visible Light1 or B) by enzymatic reaction (horseradish peroxidase, tyrosinase or laccase)2 creating a viscoelastic gelatin network. Degree of modification (number of phenolic side groups) and molecular weight can be tuned for different biomedical applications.
Need a custom or GMP biomaterial?
OR a research or technical grade gelatin?
Applications of X-Pure GelDAT
The ultra-high purity, combined with the abilty to crosslink with tissues, makes X-Pure GelDAT hydrogels ideal for research and preclinical development in:
View our customer product pipeline to see the X-Pure range of products currently in development.
Advantages of X-Pure GelDAT
Consistency, Purity and Scalability:
X-Pure GelDAT was developed to overcome the challenges researchers face when using gelatin tyramine (GelTYR).
GelTYR | X-Pure GelDAT | |
| Phenol-modified gelatin | ✓ | ✓ |
| Photo crosslinking | ✓ | ✓ |
| Enzymatic crosslinking | ✓ | ✓ |
| Controllable synthesis reaction | X | ✓ |
| Full control on molecular weight during synthesis | X | ✓ |
| Tunable rheological properties | X | ✓ |
| Purified | X | ✓ |
| Scalable | X | ✓ |
Figure 2: GelTYR production creates uncontrollable chemical side-reactions causing product loss, degradation and potential toxicological risks3, 4. Furthermore, it is not suitable for production at scale in a controllable and consistent manner, meaning synthesis and purification are almost impossible at industrial scale. X-Pure GelDAT overcomes these issues, helping to reduce clinical translation time.
Flexibility and tunability:
Like X-Pure GelMA, X-Pure GelDAT modified gelatin is available in different molecular weights and degrees of modification*, which enables the creation of hydrogels with tunable rheological properties5 and biodegradation kinetics, suitable for culturing various types of cells at body temperature.
Other advantages
These benefits are complemented by the wide range of advantages that come with Rousselot biomaterials, including:
- Batch-to-batch consistency
- Full traceability
- Research grade biomaterials are functionally similar to GMP grade, removing the need to fully re-validate pre-clinical research results prior to clinical trials
These qualities all contribute to faster translation to the clinic.
Visit our Product Finder to find the optimal biomaterial for your needs.
References
1. Al-Abboodi A, Zhang S, Al-Saady M, Ong JW, Chan PPY, Fu J. Printing in situ tissue sealant with visible-light-crosslinked porous hydrogel. Biomed Mater. 2019 May 17;14(4):045010.
2. Jeon Il Kanga and Kyung Min Park. Advances in gelatin-based hydrogels for wound management. J. Mater. Chem. B, 2021,9, 1503-1520
3. Mojarradi, H. (2010). Coupling of substances containing a primary amine to hyaluronan via carbodiimide-mediated amidation (Dissertation). Retrieved from urn.kb.se/resolve
4. Kyle A. Totaro, Xiaoli Liao, Keshab Bhattacharya, Jari I. Finneman, Justin B. Sperry, Mark A. Massa, Jennifer Thorn, Sa V. Ho, and Bradley L. Pentelute. Systematic Investigation of EDC/sNHS-Mediated Bioconjugation Reactions for Carboxylated Peptide Substrates. Bioconjugate Chemistry 2016 27 (4), 994-1004
5. Van Den Bulcke, A. I., Bogdanov, B., De Rooze, N., Schacht, E. H., Cornelissen, M., & Berghmans, H. (2000). Structural and Rheological Properties of Methacrylamide Modified Gelatin Hydrogels. Biomacromolecules,2000, 1(1), 31-38.